Article
Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica.
Yeast (Chichester, England) - 1 Jan 1991
Fournier P, Guyaneux L, Chasles M, Gaillardin C
Abstract excerpt
Previous attempts is isolate autonomously replicating sequences (ars) from the dimorphic yeast Yarrowia lipolytica have been unsuccessful. We isolated a Fil- mutant unable to produce hyphae and growing only in a yeast form to facilitate ars isolation. This mutant was transformed with a Y. lipolytica DNA bank and several unstable clones were obtained. Extrachromosomal plasmids were evidenced in yeast, recovered in...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Probes
- DNA Replication
- DNA, Fungal
- Gene Library
- Mutation
- Plasmids
- Restriction Mapping
- Saccharomycetales
- Transformation, Genetic
