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Article

An orthogonal DNA replication system in yeast.

Nature chemical biology - 1 Mar 2014

Ravikumar Arjun, Arrieta Adrian, Liu Chang C

Abstract excerpt

An extranuclear replication system, consisting of an orthogonal DNA plasmid-DNA polymerase pair, was developed in Saccharomyces cerevisiae. Engineered error-prone DNA polymerases showed complete mutational targeting in vivo: per-base mutation rates on the plasmid were increased substantially and remained stable with no increase in genomic rates. Orthogonal replication serves as a platform for in vivo continuous...

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PMID: 24487693
DOI: 10.1038/nchembio.1439

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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