Article
A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli.
Journal of microbiological methods - 1 Mar 2007
Liang Rubing, Liu Xipeng, Liu Jianhua, Ren Qiushi, Liang Peiji, Lin Zhixin, Xie Xiangming
Abstract excerpt
Temperature-sensitive (TS) mutants of a gene are ones of which the activity or phenotype is very similar to that of wild type only at certain temperature and they provide extremely powerful tool for studying protein function in vivo. Here we report a novel strategy to generate TS phenotype of the interest gene in Escherichia coli based on a temperature-sensitive T7-expression system. A TS T7-RNA polymerase is...
Topics
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Inteins
- Lac Operon
- Microbiological Techniques
- Mutation
- Phenotype
- Reverse Transcriptase Polymerase Chain Reaction
- Saccharomyces cerevisiae