Article
Identification of the pheS5 mutation, which causes thermosensitivity of Escherichia coli mutant NP37.
Journal of bacteriology - 1 Mar 1992
Kast P, Keller B, Hennecke H
Abstract excerpt
The pheS5 mutation responsible for the thermosensitive phenylalanyl-tRNA synthetase of the classical Escherichia coli NP37 was cloned by a recombination event and identified by DNA sequence analysis. The mutation was subsequently verified by direct sequencing of amplified NP37 DNA generated by an asymmetric polymerase chain reaction. The resulting amino acid exchange, Gly-98 to Asp-98 in the phenylalanyl-tRNA...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
