Article
The temperature sensitivity of a mutation in the essential tRNA modification enzyme tRNA methyltransferase D (TrmD).
The Journal of biological chemistry - 4 Oct 2013
Masuda Isao, Sakaguchi Reiko, Liu Cuiping, Gamper Howard, Hou Ya-Ming
Abstract excerpt
Conditional temperature-sensitive (ts) mutations are important reagents to study essential genes. Although it is commonly assumed that the ts phenotype of a specific mutation arises from thermal denaturation of the mutant enzyme, the possibility also exists that the mutation decreases the enzyme activity to a certain level at the permissive temperature and aggravates the negative effect further upon temperature...
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