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Article

Probing the effects of cysteine residues on protein adsorption onto gold nanoparticles using wild-type and mutated GB3 proteins.

Langmuir : the ACS journal of surfaces and colloids - 3 Sept 2013

Siriwardana Kumudu, Wang Ailin, Vangala Karthikeshwar, Fitzkee Nicholas, Zhang Dongmao

Abstract excerpt

The role of cysteine residues in the protein binding kinetics and stability on gold nanoparticles (AuNP) was studied using AuNP localized surface plasmon resonance (LSPR) in combination with an organothiol (OT) displacement method. GB3, the third IgG-binding domain of protein G, was used to model protein-AuNP adsorption. While wild-type GB3 (GB30) contains no cysteine residues, bioengineered GB3 variants...

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PMID: 23927741
DOI: 10.1021/la402239h

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