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Article

Control of a tyrosyl radical mediated protein cross-linking reaction by electrostatic interaction.

Bioconjugate chemistry - 15 Aug 2012

Minamihata Kosuke, Goto Masahiro, Kamiya Noriho

Abstract excerpt

Herein, we demonstrate the control of protein heteroconjugation via a tyrosyl coupling reaction by using electrostatic interaction. Aspartic acid and arginine were introduced to a tyrosine containing peptide tag (Y-tag) to provide electrostatic charge. Designed negatively or positively charged Y-tags were tethered to the C-terminus of Escherichia coli alkaline phosphatase (BAP) and streptavidin (SA), and these...

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Identifiers

PMID: 22816877
DOI: 10.1021/bc300137s

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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