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Article

Immobilization of immunoglobulin-G-binding domain of Protein A on a gold surface modified with biotin ligase.

Analytical biochemistry - 1 Sept 2015

Miyao Hiroki, Ikeda Yusuke, Shiraishi Arata, Kawakami Yuji, Sueda Shinji

Abstract excerpt

Protein A from Staphylococcus aureus specifically binds to the Fc region of immunoglobulin G (IgG) and is widely used as a scaffold for the immobilization of IgG antibodies on solid supports. It is known that the oriented immobilization of Protein A on solid supports enhances its antibody-binding capability in comparison with immobilization in a random manner. In the current work, we developed a novel method for...

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Identifiers

PMID: 25998102
DOI: 10.1016/j.ab.2015.05.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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