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Article

Molecular analysis of complex human cell populations: mutational spectra of MNNG and ICR-191.

Mutation research - 1 Aug 1990

Cariello N F, Keohavong P, Kat A G, Thilly W G

Abstract excerpt

We describe a method to identify and enumerate mutants at the nucleotide level in complex cell populations. Several thousand different mutants were induced at the HPRT locus in human lymphoblastoid cultures by either MNNG, an alkylating agent, or by ICR-191, a substituted acridine. HPRT mutants were selected en masse by resistance to 6-thioguanine. The most frequent mutations (hotspots) in HPRT exon 3 were...

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PMID: 2385237
DOI: 10.1016/0027-5107(90)90023-w

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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