Article
Molecular analysis of complex human cell populations: mutational spectra of MNNG and ICR-191.
Mutation research - 1 Aug 1990
Cariello N F, Keohavong P, Kat A G, Thilly W G
Abstract excerpt
We describe a method to identify and enumerate mutants at the nucleotide level in complex cell populations. Several thousand different mutants were induced at the HPRT locus in human lymphoblastoid cultures by either MNNG, an alkylating agent, or by ICR-191, a substituted acridine. HPRT mutants w...
Topics
- Aminacrine
- Aminoacridines
- B-Lymphocytes
- Base Sequence
- Cell Line
- Cloning, Molecular
- Electrophoresis
- Exons
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Methylnitronitrosoguanidine
- Molecular Sequence Data
- Mutation
- Nitrogen Mustard Compounds
- Polymerase Chain Reaction