Article
Molecular analysis of mutations in the human HPRT gene.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Keohavong Phouthone, Xi Liqiang, Grant Stephen G
Abstract excerpt
The HPRT assay uses incorporation of toxic nucleotide analogues to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosphoribosyl transferase. A major advantage of this assay is the ability to isolate mutant cells and determine the molecular basis for their functional deficiency. Many types of analyses have been performed at this locus: the current protocol involves generation of a cDNA...
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