Mismatch repair deficient human cells: spontaneous and MNNG-induced mutational spectra in the HPRT gene.

doi:10.1016/s0027-5107(00)00020-8

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Article

Mismatch repair deficient human cells: spontaneous and MNNG-induced mutational spectra in the HPRT gene.

Mutation research - 30 May 2000

Tomita-Mitchell A, Kat A G, Marcelino L A, Li-Sucholeiki X C, Goodluck-Griffith J, Thilly W G

Abstract excerpt

We have determined both the spontaneous and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutational spectra in the HPRT gene of human cells (MT1) defective in the mismatch repair gene hMSH6 (GTBP). Eight of nine exons and nine of sixteen intronic flanking sequences were scanned, encompassing >900 bp of the HPRT gene. Mutant hotspots were detected and separated by differences in their melting temperatures...

Topics

Alkylating Agents
Base Pair Mismatch
Base Sequence
Cell Line
DNA Primers
DNA Repair
DNA-Binding Proteins
Humans
Hypoxanthine Phosphoribosyltransferase
Methylnitronitrosoguanidine
Mutation
Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 10838138
DOI: 10.1016/s0027-5107(00)00020-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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