Article
Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Koletzki Diana, Pattery Theresa, Fevery Bart, Vanhooren Leen, Stuyver Lieven J
Abstract excerpt
Genotypic testing based on subtype-specific amplification and population Sanger sequencing for two nonstructural (NS) protein-coding regions, the NS3/4A protease and the NS5B polymerase, of the hepatitis C virus (HCV) genome is described here. The protocols include the molecular steps for RNA extraction, one-step RT-PCR followed by inner PCR and population Sanger sequencing, to obtain the sequence information of...
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