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Article

An NS5A single optimized method to determine genotype, subtype and resistance profiles of Hepatitis C strains.

PloS one - 1 Jan 2017

Andre-Garnier Elisabeth, Besse Bernard, Rodallec Audrey, Ribeyrol Olivier, Ferre Virginie, Luco Caroline, Le Guen Laura, Bourgeois Nathalie, Gournay Jérôme, Billaud Eric, Raffi François, Coste-Burel Marianne, Imbert-Marcille Berthe-Marie

Abstract excerpt

The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order to validate the use of a unique RT-PCR for genotypes 1-5, 142 plasma samples from patients infected with HCV were analysed. The NS4B-NS5A partial region was successfully amplified and sequenced in all samples. In parallel, partial NS3 sequences were analyzed...

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Share to Antiviral Agents
Share to Drug Resistance, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Hepacivirus

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Share to Sequence Analysis, DNA

Share to Viral Nonstructural Proteins

Source

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Identifiers

PMID: 28727784
DOI: 10.1371/journal.pone.0179562
PMCID: PMC5519038

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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