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Article

Improving the activity of cytochrome P450 BM-3 catalyzing indole hydroxylation by directed evolution.

Applied biochemistry and biotechnology - 1 Sept 2013

Pengpai Zhang, Sheng Hu, Lehe Mei, Yinlin Lei, Zhihua Jin, Guixiang Hu

Abstract excerpt

Cytochrome P450 BM-3 (A74G/F87V/L188Q) could catalyze indole to produce indigo. To further improve this capability, random mutagenesis was performed on the heme domain of P450 BM-3 (A74G/F87V/L188Q) with error-prone PCR. A single mutant V445A was selected out from the error-prone library and exhibited the highest specific activity toward indole among the mutants obtained. The kinetic parameters of V445A were also...

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PMID: 23817788
DOI: 10.1007/s12010-013-0353-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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