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Article

Directed-evolution analysis of human cytochrome P450 2A6 for enhanced enzymatic catalysis.

Journal of toxicology and environmental health. Part A - 1 Jan 2014

Lee Hwayoun, Kim Joo-Hwan, Han Songhee, Lim Young-Ran, Park Hyoung-Goo, Chun Young-Jin, Park Sung-Woo, Kim Donghak

Abstract excerpt

Cytochrome P450 2A6 (P450 2A6) is the major enzyme responsible for the oxidation of coumarin, nicotine, and tobacco-specific nitrosamines in human liver. In this study, the catalytic turnover of coumarin oxidation was improved by directed-evolution analysis of P450 2A6 enzyme. A random mutant library was constructed using error-prone polymerase chain reaction (PCR) of the open reading frame of the P450 2A6 gene...

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Identifiers

PMID: 25343290
DOI: 10.1080/15287394.2014.951757

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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