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Article

Semi-rational Directed Evolution of Monoamine Oxidase for Kinetic Resolution of rac-Mexiletine.

Applied biochemistry and biotechnology - 1 Aug 2015

Chen Zhenming, Ma Yuanhui, He Mengyan, Ren Hongyang, Zhou Shuo, Lai Dunyue, Wang Zhiguo, Jiang Linshu

Abstract excerpt

Semi-rational directed evolution was applied to the D5 variant of monoamine oxidase from Aspergillus niger (MAO-N-D5) with the aim of deriving the more desirable (R)-mexiletine through the kinetic resolution of mexiletine enantiomers. Although MAO-N-D5 shows no activity towards rac-mexiletine, theoretical molecular docking studies revealed the potential binding conformations of both mexiletine enantiomers and...

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PMID: 26093614
DOI: 10.1007/s12010-015-1716-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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