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Article

Identification of critical residues in novel drug metabolizing mutants of cytochrome P450 BM3 using random mutagenesis.

Journal of medicinal chemistry - 8 Feb 2007

van Vugt-Lussenburg Barbara M A, Stjernschantz Eva, Lastdrager Jeroen, Oostenbrink Chris, Vermeulen Nico P E, Commandeur Jan N M

Abstract excerpt

Previously, we've described a site-directed triple mutant of cytochrome P450 BM3 (BM3) that is able to convert various drugs (van Vugt-Lussenburg, B. M. A., et al. Biochem. Biophys. Res. Commun. 2006, 346, 810-818). In the present study, random mutagenesis was used to improve the activity of this mutant. With three generations of error-prone PCR, mutants were obtained with 200-fold increased turnover toward drug...

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PMID: 17266197
DOI: 10.1021/jm0609061

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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