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Article

Mutations of toluene-4-monooxygenase that alter regiospecificity of indole oxidation and lead to production of novel indigoid pigments.

Applied and environmental microbiology - 1 Sept 2005

McClay Kevin, Boss Corinne, Keresztes Ivan, Steffan Robert J

Abstract excerpt

Broad-substrate-range monooygenase enzymes, including toluene-4-monooxygenase (T4MO), can catalyze the oxidation of indole. The indole oxidation products can then condense to form the industrially important dye indigo. Site-directed mutagenesis of T4MO resulted in the creation of T4MO isoforms with altered pigment production phenotypes. High-pressure liquid chromatography, thin-layer chromatography, and nuclear...

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Identifiers

PMID: 16151140
DOI: 10.1128/AEM.71.9.5476-5483.2005
PMCID: PMC1214665

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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