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Article

[Increasing activity of a monoamine oxidase by random mutation].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jan 2014

Chen Xuejun, Ma Yuanhui, Shao Jianhua, Lai Dunyue, Wang Zhiguo, Chen Zhenming

Abstract excerpt

The monoamine oxidase mutant A-1 (F210V/L213C) from Aspergillus niger showed some catalytic activity on mexiletine. To futher improve its activity, the mutant was subjected to directed evolution with MegaWHOP PCR (Megaprimer PCR of Whole Plasmid) and selection employing a high-throughput agar plate-based colorimetric screen. This approach led to the identification of a mutant ep-1, which specific activity was...

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Identifiers

PMID: 24818485

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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