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Directed Evolution of Fluorescent Proteins in Bacteria.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Mattson Sara, Tran Geraldine N, Rodriguez Erik A

Abstract excerpt

Directed evolution has revolutionized the way scientists create new biomolecules not found in nature. Error-prone polymerase chain reaction (PCR) introduces random mutations and was used to evolve jellyfish and coral fluorescent proteins in bacteria. We describe a novel method for the directed evolution of a far-red fluorescent protein in E. coli. The new method used genes to produce fluorophores inside E. coli...

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PMID: 36107338
DOI: 10.1007/978-1-0716-2667-2_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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