Article
The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion.
Gene - 1 Mar 1992
Barnes W M
Abstract excerpt
KlenTaq DNA polymerase is an N-terminally truncated Thermus aquaticus (Taq) DNA polymerase I. As expressed from a gene construct in Escherichia coli, translation initiates at Met236, bypassing the 5'----3' exonuclease domain of the DNA polymerase-encoding gene. A sensitive forward mutation assay was used to measure the relative number of mutations introduced into the entire lacZ gene by the polymerase chain...
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