The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion.

doi:10.1016/0378-1119(92)90299-5

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Article

The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion.

Gene - 1 Mar 1992

Barnes W M

Abstract excerpt

KlenTaq DNA polymerase is an N-terminally truncated Thermus aquaticus (Taq) DNA polymerase I. As expressed from a gene construct in Escherichia coli, translation initiates at Met236, bypassing the 5'----3' exonuclease domain of the DNA polymerase-encoding gene. A sensitive forward mutation assay...

Topics

Base Sequence
Cloning, Molecular
DNA, Recombinant
DNA-Directed DNA Polymerase
Lac Operon
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Taq Polymerase
Thermus
beta-Galactosidase

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