Article
One-step cloning of intron-containing hairpin RNA constructs for RNA interference via isothermal in vitro recombination system.
Planta - 1 Aug 2013
Jiang Yanxiang, Xie Min, Zhu Qinlong, Ma Xinliang, Li Xiaoyu, Liu Yaoguang, Zhang Qunyu
Abstract excerpt
Hairpin RNA-based RNA interference (hpRNAi) has become a powerful tool for exploring gene function in reverse genetics. Although, several methods are available for making constructs that express hpRNAi, multiple time-consuming cloning steps are usually involved. Here, we introduce an efficient and flexible hpRNAi vector construction method via the isothermal in vitro recombination system (IR-hpRNAi). For an...
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