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Article

Novel constructs for efficient cloning of sRNA-encoding DNA and uniform silencing of plant genes employing artificial trans-acting small interfering RNA.

Plant cell reports - 1 Oct 2016

Baykal Ulku, Liu Hua, Chen Xinlu, Nguyen Henry T, Zhang Zhanyuan J

Abstract excerpt

KEY MESSAGE: TAS atasiRNA-producing region swapping used one-step, high efficiency, and high fidelity directional TC-cloning. Uniform silencing was achieved without lethality using miRNA trigger- TAS overexpression fusion cassettes to generate 21-nt atasiRNA. Plant transgenic technologies are very important for basic plant research and biotechnology. Artificial trans-acting small interfering RNA (atasiRNA)...

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Source

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PMID: 27417696
DOI: 10.1007/s00299-016-2024-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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