Recovery of RNA-dependent RNA polymerase 6 gene-knockout phenotypes in Nicotiana benthamiana via in vivo generation of inverted repeat construct of the trans-acting short interference RNA3 sequence.

Abstract excerpt

Pharmaceutical recombinant proteins are widely produced using plants; however, several challenges such as low productivity limit their application. To overcome these problems, RNA-dependent RNA polymerase 6-knockout Nicotiana benthamiana plants (rdr6 plants) were previously produced for the mass production of recombinant proteins. These rdr6 plants produced higher amounts of recombinant proteins than wild-type...

Topics

• Nicotiana
• RNA-Dependent RNA Polymerase
• Plants, Genetically Modified
• MicroRNAs
• Phenotype
• Plant Proteins