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In-locus gene silencing in plants using genome editing.

The New phytologist - 1 Sept 2024

Shen Rundong, Yao Qi, Tan Xinhang, Ren Wendan, Zhong Dating, Zhang Xuening, Li Xinbo, Dong Chao, Cao Xuesong, Tian Yifu, Zhu Jian-Kang, Lu Yuming

Abstract excerpt

Gene silencing is crucial in crop breeding for desired trait development. RNA interference (RNAi) has been used widely but is limited by ectopic expression of transgenes and genetic instability. Introducing an upstream start codon (uATG) into the 5'untranslated region (5'UTR) of a target gene may 'silence' the target gene by inhibiting protein translation from the primary start codon (pATG). Here, we report an...

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PMID: 38798233
DOI: 10.1111/nph.19856

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