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Article

A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants.

PloS one - 1 Jan 2014

Binder Andreas, Lambert Jayne, Morbitzer Robert, Popp Claudia, Ott Thomas, Lahaye Thomas, Parniske Martin

Abstract excerpt

The Golden Gate (GG) modular assembly approach offers a standardized, inexpensive and reliable way to ligate multiple DNA fragments in a pre-defined order in a single-tube reaction. We developed a GG based toolkit for the flexible construction of binary plasmids for transgene expression in plants. Starting from a common set of modules, such as promoters, protein tags and transcribed regions of interest, synthetic...

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Identifiers

PMID: 24551083
DOI: 10.1371/journal.pone.0088218
PMCID: PMC3923767

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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