Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants.

The New phytologist - 1 Jul 2010

Xu Guoyong, Sui Ning, Tang Yang, Xie Ke, Lai Yizhen, Liu Yule

Abstract excerpt

*The hairpin-based RNA interference (RNAi) technique plays an important role in exploring gene function in plants. Although there are several methods for making hairpin RNA (hpRNA) constructs, these methods usually need multiple relatively laborious, time-consuming or high-cost cloning steps. Here we describe a one-step, zero-background ligation-independent cloning (OZ-LIC) method for making intron-containing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Silencing
Share to Glucosyltransferases
Share to Glucuronidase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Nicotiana

Share to Nucleic Acid Conformation

Share to Rhizobium

Share to RNA Interference

Share to RNA, Plant

Source

Identifiers

PMID: 20406406
DOI: 10.1111/j.1469-8137.2010.03253.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants.PMID 20406406Read ↗

One-step cloning of intron-containing hairpin RNA constructs for RNA interference via isothermal in vitro recombination system.Semantic proximity Read ↗

Novel constructs for efficient cloning of sRNA-encoding DNA and uniform silencing of plant genes employing artificial trans-acting small interfering RNA.Semantic proximity Read ↗

In-locus gene silencing in plants using genome editing.Semantic proximity Read ↗

A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants.Semantic proximity Read ↗

Functional analysis of four members of the PsbP family in photosystem II in Nicotiana tabacum using differential RNA interference.Semantic proximity Read ↗

Tight regulation of plant immune responses by combining promoter and suicide exon elements.Semantic proximity Read ↗

Modification of endogenous natural genes by gene targeting in rice and other higher plants.Semantic proximity Read ↗

DNA elements reducing transcriptional gene silencing revealed by a novel screening strategy.Semantic proximity Read ↗

Efficient Deletion of Transgenic DNA from Complex Integration Locus of Rice Mediated by Cre/lox Recombination SystemSemantic proximity Read ↗

Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning.Semantic proximity Read ↗

TADEA-PCR is a highly efficient method of amplifying unknown flanking fragments of T-DNA transformants.Semantic proximity Read ↗

Are gene silencing mutants good tools for reliable transgene expression or reliable silencing of endogenous genes in plants?Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.