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Article

Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA.

ACS synthetic biology - 16 Aug 2013

Mitchell Leslie A, Cai Yizhi, Taylor Martin, Noronha Anne Marie, Chuang James, Dai Lixin, Boeke Jef D

Abstract excerpt

Multichange ISOthermal (MISO) mutagenesis is a new technique allowing simultaneous introduction of multiple site-directed mutations into plasmid DNA by leveraging two existing ideas: QuikChange-style primers and one-step isothermal (ISO) assembly. Inversely partnering pairs of QuikChange primers results in robust, exponential amplification of linear fragments of DNA encoding mutagenic yet homologous ends. These...

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Identifiers

PMID: 23654272
DOI: 10.1021/sb300131w
PMCID: PMC4040258

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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