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Article

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.

Analytical biochemistry - 15 Apr 2008

Tseng Wen-Chi, Lin Jing-Wei, Wei Tsen-Yun, Fang Tsuei-Yun

Abstract excerpt

In this study, we report a novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method modified from the QuikChange site-directed mutagenesis (QCM). One mutagenic oligonucleotide and one universal flanking primer were used to produce the complementary megaprimers that were then used to amplify the whole plasmid template. This method yields a mutagenesis efficiency ( approximately 90%) similar to...

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Identifiers

PMID: 18198125
DOI: 10.1016/j.ab.2007.12.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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