Article
Lectin-resistant CHO cells: selection of seven new mutants resistant to ricin.
Somatic cell and molecular genetics - 1 May 1990
Stanley P, Sallustio S, Krag S S, Dunn B
Abstract excerpt
In attempts to isolate new CHO glycosylation mutants, selection protocols using plant lectins that bind galactose residues of cell surface carbohydrates were applied to mutagenized CHO populations. The lectins were used alone or in combination to obtain seven ricin-resistant phenotypes. Each mutant had distinctive properties compared with previously described ricin-resistant CHO cells. One of the new phenotypes...
Topics
- Animals
- Cell Line
- Cricetinae
- Galactose
- Genetic Complementation Test
- Genotype
- Glycosylation
- Lectins
- Mutation
- Phenotype
- Ricin