Article
A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant.
Biochemistry - 28 Oct 2003
Lee JaeHoon, Park Sung-Hae, Sundaram Subha, Raju T Shantha, Shaper Nancy L, Stanley Pamela
Abstract excerpt
To identify factors required for the synthesis of complex glycans, we have isolated Chinese hamster ovary (CHO) cell mutants resistant to plant lectins. We previously identified Lec19 CHO cells as resistant to the Gal-binding lectins ricin, abrin, and modeccin and hypersensitive to the toxicity of other lectins that bind Gal, including L-PHA and E-PHA. Here we show that Lec19 cell extracts have a decreased...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.