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Article

Improved loop-mediated isothermal amplification for HLA-DRB1 genotyping using RecA and a restriction enzyme for enhanced amplification specificity.

Immunogenetics - 1 Jun 2013

Mitsunaga Shigeki, Shimizu Sayoko, Okudaira Yuko, Oka Akira, Tanaka Masafumi, Kimura Minoru, Kulski Jerzy K, Inoue Ituro, Inoko Hidetoshi

Abstract excerpt

Our aim was to test and develop the use of loop-mediated isothermal amplification (LAMP) for HLA-DRB1 genotyping. Initially, we found that the conventional LAMP protocols produced non-specific and variable amplification results depending on the sample DNA conditions. Experiments with different concentrations of DNase in the reaction mixture with and without T4 DNA ligase-treated samples suggested that the strand...

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PMID: 23474534
DOI: 10.1007/s00251-013-0690-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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