Article
High-efficiency and high-fidelity ssDNA circularisation via the pairing of five 3'-terminal bases to assist LR-LAMP for the genotyping of single-nucleotide polymorphisms.
The Analyst - 12 Sept 2022
Li Taiwen, Zou Huayan, Zhang Jing, Ding Haixia, Li Cheng, Chen Xiangru, Li Yunzhou, Feng Wenzhuo, Kageyama Koji
Abstract excerpt
The poor fidelity of T4 DNA ligase has always limited the simple detection of single-nucleotide polymorphisms (SNPs) and is only applicable to some special SNP types. This study developed a highly sensitive and specific detection method for SNPs based on high-fidelity single-stranded circularisation. It used T4 DNA ligase and rolling circle amplification (RCA) plus loop-mediated isothermal amplification (LAMP)....
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