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Article

Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR.

Scientific reports - 13 Jan 2015

Ghindilis Andrey L, Smith Maria W, Simon Holly M, Seoudi Ihab A, Yazvenko Nina S, Murray Iain A, Fu Xiaoqing, Smith Kenneth, Jen-Jacobson Linda, Xu Shuang-Yong

Abstract excerpt

An alternative to qPCR was developed for nucleic acid assays, involving signal rather than target amplification. The new technology, Restriction Cascade Exponential Amplification (RCEA), relies on specific cleavage of probe-target hybrids by restriction endonucleases (REase). Two mutant REases fo...

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Identifiers

PMID: 25583452
DOI: 10.1038/srep07737
PMCID: PMC4291554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25583452-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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