Article
Recombinase assisted loop-mediated isothermal DNA amplification.
The Analyst - 21 Jan 2020
Chen Gangyi, Chen Rong, Ding Sheng, Li Mei, Wang Jiayu, Zou Jiawei, Du Feng, Dong Juan, Cui Xin, Huang Xin, Deng Yun, Tang Zhuo
Abstract excerpt
Polymerase chain reaction (PCR) and isothermal amplification methods such as LAMP and RPA are widely used for genetic detection. However, there are some shortcomings of these methods such as dependence on thermocycler instruments for PCR, complexity of primer design, the possibility for nonspecific amplification in LAMP and complexity of components in RPA. We develop a novel isothermal DNA detection system named...
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