Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

beta-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris.

Letters in applied microbiology - 1 Nov 1999

Ayra-Pardo C, Montejo-Sierra I L, Vázquez-Padrón R I, García-Martínez C

Abstract excerpt

The Escherichia coli gene gusA was expressed in the methylotrophic yeast Pichia pastoris in a transcriptional fusion to the homologous methanol-inducible AOX1 promoter. Four recombinant clones were selected for expression studies in shake flask conditions and beta-D-glucuronidase (beta-GUS) activity was assayed each 24 h during the induction period. Regardless of the genomic integration patterns and the gene...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Southern
Share to Genes, Reporter

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Glucuronidase

Share to Mitochondrial Proteins

Share to Oxidoreductases

Share to Pichia

Share to Recombinant Fusion Proteins

Share to Transformation, Genetic

Source

Identifiers

PMID: 10664965
DOI: 10.1046/j.1365-2672.1999.00610.x

Genes

AOX1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

beta-D-glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris.PMID 10664965Read ↗

An episomal expression vector for screening mutant gene libraries in Pichia pastoris.Semantic proximity Read ↗

Recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris.Semantic proximity Read ↗

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway.Semantic proximity Read ↗

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris.Semantic proximity Read ↗

Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.Semantic proximity Read ↗

Evaluation of Mut(S) and Mut⁺ Pichia pastoris strains for membrane-bound catechol-O-methyltransferase biosynthesis.Semantic proximity Read ↗

Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris.Semantic proximity Read ↗

Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures.Semantic proximity Read ↗

Pichia pastoris Mut(S) strains are prone to misincorporation of O-methyl-L-homoserine at methionine residues when methanol is used as the sole carbon source.Semantic proximity Read ↗

Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.