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Article

Generation of bovine intraspecies hybridomas with initial suppressed growth.

Veterinary immunology and immunopathology - 1 Mar 1990

Olobo J O, Black S J

Abstract excerpt

Bovine B-cell hybrids were generated by fusing pokeweed mitogen (PWM) activated normal bovine peripheral blood lymphocytes (PBL) with an aminopterin-sensitive bovine B-cell line BL20. The fusion partner was derived by irradiation and growth in medium containing increasing concentrations of 6-thioguanine (6TG). Resultant cells were designated ATS/BL20. Polyethylene glycol-induced (PEG) fusion was used and hybrids...

Topics

Aminopterin
Animals
Antigens, Surface
B-Lymphocytes
Cattle
Cell Division
Cell Line
Cell Survival
Culture Media
Hybrid Cells
Immunoglobulin G
Immunoglobulin M

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Karyotyping

Mutation

Source

Identifiers

PMID: 2339500
DOI: 10.1016/0165-2427(90)90044-s

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2339500-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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