Article
Application of an immune-tolerizing procedure to generate monoclonal antibodies specific to an alternate protein isoform of bovine growth hormone.
Analytical biochemistry - 15 Nov 1992
Salata R A, Malhotra I J, Hampson R K, Ayers D F, Tomich C S, Rottman F M
Abstract excerpt
An immune-tolerizing protocol was employed to generate monoclonal antibodies to a variant protein isoform of bovine growth hormone arising from alternative pre-mRNA processing. Variant bovine growth hormone used for immunization was obtained by expression in bacteria and electroelution of the protein from preparative sodium dodecyl sulfate-polyacrylamide gels. Balb/c mice were first immunized with wild-type...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Cattle
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Genetic Variation
- Growth Hormone
- Haplorhini