Article
Establishment of a human B cell line that responds specifically to B cell growth factor.
Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao - 1 Jan 1990
Zhu L P, Shi L, Zheng D I, Guo B C, Wang X, Zhang S Z
Abstract excerpt
A human B cell line (3D5) that responds specifically to B cell growth factor (BCGF) has been developed by a sequence of Staphylococcus aureus Cowen I activation, EB virus immortalization, and cloning. Proliferative response to PHA-stimulated T cell supernatant (PHA-T-Sup) and nonresponsiveness to rIL-2 stimulation were factors used to screen positive cells. Phenotype analysis with a flow cytometer indicated that:...
Topics
- B-Lymphocytes
- Cell Division
- Cell Line
- Flow Cytometry
- Humans
- Interleukin-4
- Phenotype