Article
Active site-directed double mutants of dihydrofolate reductase.
Cancer research - 15 Sept 1996
Ercikan-Abali E A, Mineishi S, Tong Y, Nakahara S, Waltham M C, Banerjee D, Chen W, Sadelain M, Bertino J R
Abstract excerpt
Variants of dihydrofolate reductase (DHFR), which confer resistance to antifolates, are used as dominant selectable markers in vitro and in vivo and may be useful in the context of gene therapy. To identify improved mutant human DHFRs with increased catalytic efficiency and decreased binding to methotrexate, we constructed by site-directed mutagenesis four variants with substitutions at both Leu22 and Phe31...
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