Article
Isolation and characterization of a mutant dihydrofolate reductase-thymidylate synthase from methotrexate-resistant Leishmania cells.
The Journal of biological chemistry - 8 Apr 1994
Arrebola R, Olmo A, Reche P, Garvey E P, Santi D V, Ruiz-Perez L M, Gonzalez-Pacanowska D
Abstract excerpt
The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Science 233, 535-540). Now we report that these methotrex...
Topics
- Animals
- Cloning, Molecular
- Drug Resistance
- Electrophoresis, Gel, Two-Dimensional
- Electrophoresis, Polyacrylamide Gel
- Kinetics
- Leishmania major
- Methotrexate
- Mutation
- Tetrahydrofolate Dehydrogenase
- Thymidylate Synthase
- Transfection