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Article

Identification of methionine-containing tryptic peptides of unstable beta-tubulin separated by reverse-phase high-performance liquid chromatography.

Analytical biochemistry - 1 Jan 1990

Minotti A M, Loeb L M, Cook R, Boggs B A, Cabral F

Abstract excerpt

Methods for examining altered regions in unstable mutant proteins are described. The strategy is illustrated using assembly defective Chinese hamster beta-tubulin subunits that are rapidly degraded in the cell. These unstable proteins are metabolically labeled to high specific activity and isolat...

Topics

Amino Acid Sequence
Animals
Cell Line
Chromatography, High Pressure Liquid
Cricetinae
Female
Methionine
Mutation
Ovary
Peptide Fragments
Peptide Mapping
Trypsin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tubulin

Source

Identifiers

PMID: 2321756
DOI: 10.1016/0003-2697(90)90006-u

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2321756-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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