Article
Site-directed mutagenesis of putative GTP-binding sites of yeast beta-tubulin: evidence that alpha-, beta-, and gamma-tubulins are atypical GTPases.
Biochemistry - 6 Jun 1995
Sage C R, Dougherty C A, Davis A S, Burns R G, Wilson L, Farrell K W
Abstract excerpt
The exchangeable GTP-binding site on beta-tubulin has been extensively studied, but the primary sequence elements which form the binding site on beta-tubulin remain unknown. We have used site-directed mutagenesis of the single beta-tubulin gene of Saccharomyces cerevisiae to test a model for the...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cattle
- Consensus Sequence
- GTP Phosphohydrolases
- Genes, Fungal
- Genotype
- Guanosine Triphosphate
- Kinetics
- Microtubules
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Point Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- Tubulin