Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Resistance to antimitotic drugs in Chinese hamster ovary cells correlates with changes in the level of polymerized tubulin.

The Journal of biological chemistry - 25 Feb 1991

Minotti A M, Barlow S B, Cabral F

Abstract excerpt

A sensitive and reproducible method to measure relative levels of polymerized and soluble tubulin in cells has been developed. This method involves metabolically labeling cells with radioactive amino acids followed by lysis in a microtubule-stabilizing buffer, centrifugation to separate soluble from polymerized tubulin, resolution of the proteins in each fraction by two-dimensional gel electrophoresis, and...

Topics

Alkaloids
Animals
Autoradiography
Cells, Cultured
Cricetinae
Cricetulus
Demecolcine
Drug Resistance
Electrophoresis, Gel, Two-Dimensional
Fluorescent Antibody Technique
Microtubules

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mitosis

Mutation

Paclitaxel

Polymers

Tubulin

Source

Identifiers

PMID: 1671676

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1671676-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Resistance to antimitotic drugs in Chinese hamster ovary cells correlates with changes in the level of polymerized tubulin.PMID 1671676Read ↗

Paclitaxel-dependent mutants have severely reduced microtubule assembly and reduced tubulin synthesis.Semantic proximity Read ↗

Mutations in alpha- and beta-tubulin that stabilize microtubules and confer resistance to colcemid and vinblastine.Semantic proximity Read ↗

Paclitaxel resistance in cells with reduced beta-tubulin.Semantic proximity Read ↗

Resistance to antimitotic agents as genetic probes of microtubule structure and function.Semantic proximity Read ↗

Enhanced stability of microtubules contributes in the development of colchicine resistance in MCF-7 cells.Semantic proximity Read ↗

Intra-allelic suppression of a mutation that stabilizes microtubules and confers resistance to colcemid.Semantic proximity Read ↗

Mutations at leucine 215 of beta-tubulin affect paclitaxel sensitivity by two distinct mechanisms.Semantic proximity Read ↗

The Coral-Derived Natural Products Eleutherobin and Sarcodictyins A and B: Effects on the Assembly of Purified Tubulin with and without Microtubule-Associated Proteins and Binding at the Polymer Taxoid SiteSemantic proximity Read ↗

Paclitaxel-resistant human ovarian cancer cells have mutant beta-tubulins that exhibit impaired paclitaxel-driven polymerization.Semantic proximity Read ↗

Resistance to Taxol in lung cancer cells associated with increased microtubule dynamicsSemantic proximity Read ↗

Response of microtubules to the addition of colchicine and tubulin–colchicine: evaluation of models for the interaction of drugs with microtubulesSemantic proximity Read ↗

Amino acid substitutions at proline 220 of beta-tubulin confer resistance to paclitaxel and colcemid.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.