Article
Newly-synthesized beta-tubulin demonstrates domain-specific interactions with the cytosolic chaperonin.
Biochemistry - 10 Dec 1996
Dobrzynski J K, Sternlicht M L, Farr G W, Sternlicht H
Abstract excerpt
Tubulin folding requires two chaperone systems, i.e., the 900 kDa cytosolic chaperonin referred to as the TCP-1 complex or TRiC which facilitates folding of the alpha- and beta-tubulin subunits and a ca. 180 kDa complex which facilitates further assembly into heterodimer. beta-Tubulin mutants wer...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Chaperonin Containing TCP-1
- Chaperonins
- Chickens
- Chromatography, Gel
- Chymotrypsin
- Cytoplasm
- Electrophoresis, Polyacrylamide Gel
- Intracellular Signaling Peptides and Proteins
- Microtubule-Associated Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nuclear Proteins
- Peptide Fragments
- Precipitin Tests