Article
Site-directed insertion of long single-stranded DNA fragments into plasmid DNA.
DNA and cell biology - 1 Jan 2000
Mazin A V, Saparbaev M K, Ovchinnikova L P, Dianov G L, Salganik R I
Abstract excerpt
A new site-directed method for inserting long single-stranded DNA fragments into any region of a duplex vector is described. Its major advantage is independence of the location of the restriction sites. The method involves the assembly of single-stranded DNA fragments by ligation to both ends of the inserted fragments of two cohesive flanks that are complementary to the target region. Short oligonucleotide...
Topics
- Base Sequence
- DNA Repair
- DNA, Single-Stranded
- Genetic Engineering
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Plasmids
- Receptors, Glucocorticoid
- Recombination, Genetic
- Transfection