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Article

Site-directed insertion of long single-stranded DNA fragments into plasmid DNA.

DNA and cell biology - 1 Jan 2000

Mazin A V, Saparbaev M K, Ovchinnikova L P, Dianov G L, Salganik R I

Abstract excerpt

A new site-directed method for inserting long single-stranded DNA fragments into any region of a duplex vector is described. Its major advantage is independence of the location of the restriction sites. The method involves the assembly of single-stranded DNA fragments by ligation to both ends of the inserted fragments of two cohesive flanks that are complementary to the target region. Short oligonucleotide...

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Identifiers

PMID: 2317271
DOI: 10.1089/dna.1990.9.63

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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