A method for cloning mixtures of long, synthetic oligodeoxynucleotides.

Abstract excerpt

A method is described for cloning synthetic oligodeoxynucleotides, which can theoretically be of any length. The method requires only a single oligodeoxynucleotide strand and a vector with two unique restriction sites, one of which is for an enzyme that generates 3' protruding ends. A mixture of unpurified oligonucleotides containing a wild-type genetic regulatory sequence of the Escherichia coli gnd gene and two...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Cloning, Molecular
• Share to Genetic Vectors