Article
Solid phase in vitro mutagenesis using plasmid DNA template.
Nucleic acids research - 11 Sept 1990
Hultman T, Murby M, Ståhl S, Hornes E, Uhlén M
Abstract excerpt
Site-specific mutagenesis was accomplished using a solid support to generate single stranded vector and insert fragments which can be used to form gap-duplex plasmids through flanking, complementary double stranded regions. More than 80% mutants were obtained in both a single and a double primer approach. No special vectors or strains are needed and mismatch repair is avoided as the mutagenesis region is in a...
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