Article
Solid phase in vitro mutagenesis using plasmid DNA template.
Nucleic acids research - 11 Sept 1990
Hultman T, Murby M, Ståhl S, Hornes E, Uhlén M
Abstract excerpt
Site-specific mutagenesis was accomplished using a solid support to generate single stranded vector and insert fragments which can be used to form gap-duplex plasmids through flanking, complementary double stranded regions. More than 80% mutants were obtained in both a single and a double primer...
Topics
- Base Sequence
- Escherichia coli
- Genetic Techniques
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Templates, Genetic
- Transformation, Bacterial