Article
Determination of allele frequency in pooled DNA: comparison of three PCR-based methods.
BioTechniques - 1 Dec 2005
Wilkening Stefan, Hemminki Kari, Thirumaran Ranjit Kumar, Bermejo Justo Lorenzo, Bonn Stefan, Försti Asta, Kumar Rajiv
Abstract excerpt
Determination of allele frequency in pooled DNA samples is a powerful and efficient tool for large-scale association studies. In this study, we tested and compared three PCR-based methods for accuracy, reproducibility, cost, and convenience. The methods compared were: (i) real-time PCR with allele-specific primers, (ii) real-time PCR with allele-specific TaqMan probes, and (iii) quantitative sequencing. Allele...
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