Article
High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Heissl Angelika, Arbeithuber Barbara, Tiemann-Boege Irene
Abstract excerpt
Real-time PCR-based genotyping methods, such as TaqMan allelic discrimination assays and allele-specific genotyping, are particularly useful when screening a handful of single nucleotide polymorphisms in hundreds of samples; either derived from different individuals, tissues, or pre-amplified DNA. Although real-time PCR-based methods such as TaqMan are well-established, alternative methods, like allele-specific...
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